The study aimed to investigate the humor styles of pre-service early childhood teachers. The population of the study was comprised of pre-service teachers being trained at the Department of Early Childhood Education of Afyon Kocatepe University. A total number of 214 pre-service early childhood teachers constituted the sample for this study. To gather data, a general information form and “Humor Styles Questionnaire” developed by Martin, Puhlik-Doris, Larsen, Gray and Weir (2003) and adapted to Turkish by Yerlikaya (2003) were utilized. Mann-Whitney U test was used in order to find out whether the participants’ humor styles differed according to their genders while Kruskall-Wallis H test was used in analyzing the data to detect any differences according to the participants’ academic years of study. At the end of the research, it was revealed that significant differences existed between the pre-service teachers’ humor styles and their genders together with their years of study.