In order to achieve the targeted objectives desired level of education and modern learning theories for learnercentered methods are recommended. In this context the use of worksheets developed and that student participation is considered to be one of the methods. This research is one of the ethyl alcohol fermentation biology issues and prepare working papers related to the effective education environment was conducted to determine the effect of learning the use of worksheets. Worksheets development process to experts in the field of material in order to developed, four teachers from working in the province of Trabzon. Curriculum subject and the subject determined by considering the behavior of the target sample selected as an achievement test was developed and Bayburt Education Faculty of Bayburt University science education to about 28students that 2 grade. Later, the interviews with teachers, and achievement test results utilizing "ethyl alcohol fermentation" A study on the leaf has been drafted. At the same teachers which prepared the necessary corrections were made in the drafts discussed the applicability of learning environments. The worksheet is applied on the sample above the spring semester of 2012. Pre-prepared students for success by developing a test applied to test parallel and compared with previous results. Student achievement is rising and the course is very relevant to the act have been identified.